The Effect of Intralysosomal Sucrose Storage

1. The effect of overloading of hamster fibroblast lysosomes with sucrose on the turnover of lysosomal and Golgi-apparatus enzymes was studied. Arylsulphatase B and UDPgalactose-N-acetylglucosamine galactosyltransferase were chosen as appropriate marker enzymes. The relative contributions ofchanges in the rates of synthesis and degradation to the increased activities ofthese enzymes after uptake ofsucrose were examined by isotopiclabelling experiments. The effects of sucrose uptake on the degradation of total cellular protein and of the cytoplasmic enzyme, alkaline ribonuclease, were determined for comparative purposes. 2. The rates of enzyme synthesis in the presence and absence of